Algorithm specific parameters: 

Full window size : 21
Core window size : 11
Wedge window size: 5
Using hydrophobicity file: GES-scale

Cutoff for certain transmembrane segments: 1.00
Cutoff for putative transmembrane segments: 0.60
Critical distance between 2 transmembrane segments: 2

Critical loop length:  60

Kingdom: procaryote

Using cyt/ext file: CYTEXT-scale


Sequence : first  (75 res)
VAKLFADAGLVCITSFISPYTRRRRVAKLFADAGLVCITSFISPYTRRRRVAKLFAIIII
IIIIIIISPYTRRRR

Found: 3 segments

Candidate membrane-spanning segments:

 Helix Begin - End   Score Certainity
     1     1 - 21    0.960 Putative
     2    26 - 46    0.960 Putative
     3    51 - 71    2.310 Certain

Total of 4 structures are to be tested

HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
TOPOLOGY   1                  1.00         7.00     0.00      1.00    -0.69
TOPOLOGY                                   N-in        ?      N-in
CYT_LOOP      1     50     50             11.00  ( -0.15)
TRANSMEM     51     71     21 1.00 2.31
EXT_LOOP     72     75      4              4.00  ( -1.01)
//
HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
TOPOLOGY   2                  0.90        -6.00     0.00     -3.00     0.00
TOPOLOGY                                  N-out        ?     N-out
TRANSMEM      1     21     21 0.90 0.96
CYT_LOOP     22     50     29             10.00  ( -0.60)
TRANSMEM     51     71     21 1.00 2.31
EXT_LOOP     72     75      4              4.00  ( -1.01)
//
HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
TOPOLOGY   3                  0.90         5.00     0.00      0.00    -0.71
TOPOLOGY                                   N-in        ?         ?
CYT_LOOP      1     25     25              6.00  ( -0.15)
TRANSMEM     26     46     21 0.90 0.96
EXT_LOOP     47     50      4              5.00  ( -1.01)
TRANSMEM     51     71     21 1.00 2.31
CYT_LOOP     72     75      4              4.00  ( -1.01)
//
HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
TOPOLOGY   4                  0.90        -4.00     0.00     -3.00     0.00
TOPOLOGY                                  N-out        ?     N-out
TRANSMEM      1     21     21 0.90 0.96
CYT_LOOP     22     25      4              5.00  ( -1.01)
TRANSMEM     26     46     21 0.90 0.96
EXT_LOOP     47     50      4              5.00  ( -1.01)
TRANSMEM     51     71     21 1.00 2.31
CYT_LOOP     72     75      4              4.00  ( -1.01)
//
