\begin{Verbatim}[commandchars=\\\{\}]
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{k+kn}{from} \PYG{n+nn}{Bio} \PYG{k+kn}{import} \PYG{n}{AlignIO}
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{n}{align} \PYG{o}{=} \PYG{n}{AlignIO}\PYG{o}{.}\PYG{n}{read}\PYG{p}{(}\PYG{l+s+s2}{\PYGZdq{}needle.txt\PYGZdq{}}\PYG{p}{,} \PYG{l+s+s2}{\PYGZdq{}emboss\PYGZdq{}}\PYG{p}{)}
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{n+nb}{print}\PYG{p}{(}\PYG{n}{align}\PYG{p}{)}
\PYG{g+go}{Alignment with 2 rows and 149 columns}
\PYG{g+go}{MV\PYGZhy{}LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTY...KYR HBA\PYGZus{}HUMAN}
\PYG{g+go}{MVHLTPEEKSAVTALWGKV\PYGZhy{}\PYGZhy{}NVDEVGGEALGRLLVVYPWTQRF...KYH HBB\PYGZus{}HUMAN}
\end{Verbatim}
