\begin{Verbatim}[commandchars=\\\{\}]
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{k+kn}{import} \PYG{n+nn}{os}
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{k+kn}{from} \PYG{n+nn}{Bio.Align.Applications} \PYG{k+kn}{import} \PYG{n}{ClustalwCommandline}
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{n}{clustalw\PYGZus{}exe} \PYG{o}{=} \PYG{l+s+sa}{r}\PYG{l+s+s2}{\PYGZdq{}C:\PYGZbs{}Program Files\PYGZbs{}new clustal\PYGZbs{}clustalw2.exe\PYGZdq{}}
\PYG{g+gp}{\PYGZgt{}\PYGZgt{}\PYGZgt{} }\PYG{n}{clustalw\PYGZus{}cline} \PYG{o}{=} \PYG{n}{ClustalwCommandline}\PYG{p}{(}\PYG{n}{clustalw\PYGZus{}exe}\PYG{p}{,} \PYG{n}{infile}\PYG{o}{=}\PYG{l+s+s2}{\PYGZdq{}opuntia.fasta\PYGZdq{}}\PYG{p}{)}
\end{Verbatim}
