## PRINSEQ-graphs v0.6 HTML Report

[Generated: 11/10/2013 12:12:36]
Input Information

Input file(s): | example1.fastq
Input format(s): | FASTQ
# Sequences: | 12
Total bases: | 1,150

Length Distribution

Mean sequence length: | 95.83 ± 37.96 bp
Minimum length: | 50 bp
Maximum length: | 200 bp
Length range: | 151 bp
Mode length: | 100 bp with 8 sequences

GC Content Distribution

Mean GC content: | 48.17 ± 14.83 %
Minimum GC content: | 0 %
Maximum GC content: | 57 %
GC content range: | 58 %
Mode GC content: | 50 % with 5 sequences

Base Quality Distribution

Occurence of N

Sequences with N: | 2 (16.67 %)
Max percentage of Ns per sequence: | 10 %

Poly-A/T Tails

| 5'-end | 3'-end
Sequences with tail: | 1 (8.33 %) | 1 (8.33 %)
Maximum tail length: | 50 | 50

Tag Sequence Check

| 5'-end | 3'-end
Probability of tag sequence: | 33 % | 33 %
GSMIDs or RLMIDs: | none |

|  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  | ... |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
| 1 |  |  |  | 5 |  |  |  |  | 10 |  |  |  |  | 15 |  |  |  |  | 20 |  | 20 |  |  |  |  | 15 |  |  |  |  | 10 |  |  |  |  | 5 |  |  |  | 1
| Position from Sequence Ends

Sequence Duplication

| # Sequences | Max duplicates
Exact duplicates: | 4 (33.33 %) | 3
Exact duplicates with reverse complements: | 0 | 0
5' duplicates | 0 | 0
3' duplicates | 0 | 0
5'/3' duplicates with reverse complements | 0 | 0
Total: | 4 (33.33 %) | -

Sequence Complexity

| Value | Sequence
Minimum DUST score: | 2 | NNNNNNNNNNTACACCAGAGGTGTCTCTGTGTGGGGCCTGTGTGCCAAAAGTGAGAGTTG AGAAGAGGCGTGGAGGAGATGACACACCCCGTGTGTTCTC
Maximum DUST score: | 100 | AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Minimum Entropy value: | 0 | AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Maximum Entropy value: | 81 | NNNNNNNNNNTACACCAGAGGTGTCTCTGTGTGGGGCCTGTGTGCCAAAAGTGAGAGTTG AGAAGAGGCGTGGAGGAGATGACACACCCCGTGTGTTCTC

Dinucleotide Odds Ratios

| AA/TT | AC/GT | AG/CT | AT | CA/TG | CC/GG | CG | GA/TC | GC | TA
Odds ratio | 0.3954 | 2.4378 | 0.4858 | 0.3623 | 1.0736 | 0.3006 | 1.9166 | 0.4338 | 0.5829 | 1.8332