SEQUENCE_ID=pair_1
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=primer_repeat_boundary2_lib
PRIMER_INTERNAL_MISHYB_LIBRARY=primer_repeat_boundary2_lib
SEQUENCE_TEMPLATE=GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATATATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCGCGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGCGC
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_MAX_LIBRARY_MISPRIMING=1
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=1
PRIMER_MAX_SELF_ANY=25
PRIMER_MAX_SELF_END=25
PRIMER_PAIR_MAX_COMPL_ANY=25
PRIMER_PAIR_MAX_COMPL_END=25
PRIMER_INTERNAL_MAX_SELF_ANY=25
PRIMER_INTERNAL_MAX_SELF_END=25
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_SIZE=20
PRIMER_INTERNAL_MIN_TM=0
PRIMER_INTERNAL_MAX_SIZE=20
PRIMER_MIN_GC=0
PRIMER_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
PRIMER_INTERNAL_MAX_GC=100
PRIMER_MAX_SIZE=20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=20
PRIMER_MAX_TM=100
PRIMER_MIN_TM=0
PRIMER_OPT_TM=60
PRIMER_LEFT_EXPLAIN=considered 83, high repeat similarity 82, ok 1
PRIMER_RIGHT_EXPLAIN=considered 83, high repeat similarity 71, ok 12
PRIMER_INTERNAL_EXPLAIN=considered 163, high repeat similarity 121, ok 42
PRIMER_PAIR_EXPLAIN=considered 381, unacceptable product size 369, no internal oligo 11, ok 1
PRIMER_LEFT_NUM_RETURNED=1
PRIMER_RIGHT_NUM_RETURNED=1
PRIMER_INTERNAL_NUM_RETURNED=1
PRIMER_PAIR_NUM_RETURNED=1
PRIMER_PAIR_0_PENALTY=63.584014
PRIMER_LEFT_0_PENALTY=31.846382
PRIMER_RIGHT_0_PENALTY=31.737633
PRIMER_INTERNAL_0_PENALTY=31.846382
PRIMER_LEFT_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
PRIMER_RIGHT_0_SEQUENCE=GCGCGCGCGCGCGCCGCGCG
PRIMER_INTERNAL_0_SEQUENCE=GCGCGCGCGCGCGCGCGCGC
PRIMER_LEFT_0=0,20
PRIMER_RIGHT_0=181,20
PRIMER_INTERNAL_0=111,20
PRIMER_LEFT_0_TM=91.846
PRIMER_RIGHT_0_TM=91.738
PRIMER_INTERNAL_0_TM=91.846
PRIMER_LEFT_0_GC_PERCENT=100.000
PRIMER_RIGHT_0_GC_PERCENT=100.000
PRIMER_INTERNAL_0_GC_PERCENT=100.000
PRIMER_LEFT_0_SELF_ANY=20.00
PRIMER_RIGHT_0_SELF_ANY=14.00
PRIMER_INTERNAL_0_SELF_ANY=20.00
PRIMER_LEFT_0_SELF_END=20.00
PRIMER_RIGHT_0_SELF_END=14.00
PRIMER_INTERNAL_0_SELF_END=20.00
PRIMER_LEFT_0_LIBRARY_MISPRIMING=0.00, test_1    * 2.0
PRIMER_RIGHT_0_LIBRARY_MISPRIMING=0.00, test_1    * 2.0
PRIMER_PAIR_0_LIBRARY_MISPRIMING=0.00, test_1    * 2.0
PRIMER_INTERNAL_0_LIBRARY_MISHYB=0.00, test_1    * 2.0
PRIMER_LEFT_0_END_STABILITY=13.4000
PRIMER_RIGHT_0_END_STABILITY=13.4000
PRIMER_PAIR_0_COMPL_ANY=17.00
PRIMER_PAIR_0_COMPL_END=17.00
PRIMER_PAIR_0_PRODUCT_SIZE=182
=
SEQUENCE_ID=pair_0
P3_COMMENT="Should fail because of high repeat similarity"
PRIMER_MAX_LIBRARY_MISPRIMING=20
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=20
SEQUENCE_TEMPLATE=TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
PRIMER_LEFT_EXPLAIN=considered 64, high repeat similarity 64, ok 0
PRIMER_RIGHT_EXPLAIN=considered 64, ok 64
PRIMER_INTERNAL_EXPLAIN=considered 144, ok 144
PRIMER_PAIR_EXPLAIN=considered 32, unacceptable product size 32, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
