| extractseq

## Wiki

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Please help by correcting and extending the Wiki pages.

## Function

Extract regions from a sequence

## Description

extractseq reads a sequence and writes sub-sequences from it to file. The set of regions to extract is specified on the command-line or in a file as pairs of start and end positions. The regions are written in the order in which they are specified. Thus, if the sequence `AAAGGGTTT` has been input and the regions: `7-9, 3-4` have been specified, then the output sequence will be: `TTTAG`. Optionally, each region may be written out as a separate sequence.

## Usage

Here is a sample session with extractseq

Extract the region from position 10 to 20:

    % extractseq tembl:x65923 result.seq -regions "10-20"
    Extract regions from a sequence

Go to the input files for this example
Go to the output files for this example

Example 2

Extract the regions 10 to 20, 30 to 45, 533 to 537:

    % extractseq tembl:x65921 result2.seq -regions "10-20 30-45 533-537"
    Extract regions from a sequence

Go to the input files for this example
Go to the output files for this example

Example 3

Extract the regions 782-856, 951-1095, 1557-1612 and 1787-1912:

    % extractseq tembl:x65921 -reg "782..856,951..1095,1557..1612,1787..1912" stdout
    Extract regions from a sequence

    >X65921 X65921.1 H.sapiens fau 1 gene
    atgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacg
    gtcgcccagatcaaggctcatgtagcctcactggagggcattgccccggaagatcaagtc
    gtgctcctggcaggcgcgcccctggaggatgaggccactctgggccagtgcggggtggag
    gccctgactaccctggaagtagcaggccgcatgcttggaggtaaagtccatggttccctg
    gcccgtgctggaaaagtgagaggtcagactcctaaggtggccaaacaggagaagaagaag
    aagaagacaggtcgggctaagcggcggatgcagtacaaccggcgctttgtcaacgttgtg
    cccacctttggcaagaagaagggccccaatgccaactcttaa

Example 4

Extract the regions 782-856, 951-1095, 1557-1612 and 1787-1912 all to separate output sequences:

    % extractseq tembl:x65921 -reg "782..856,951..1095,1557..1612,1787..1912" stdout -separate
    Extract regions from a sequence

    >X65921_782_856 H.sapiens fau 1 gene
    atgcagctctttgtccgcgcccaggagctacacaccttcgaggtgaccggccaggaaacg
    gtcgcccagatcaag
    >X65921_951_1095 H.sapiens fau 1 gene
    gctcatgtagcctcactggagggcattgccccggaagatcaagtcgtgctcctggcaggc
    gcgcccctggaggatgaggccactctgggccagtgcggggtggaggccctgactaccctg
    gaagtagcaggccgcatgcttggag
    >X65921_1557_1612 H.sapiens fau 1 gene
    gtaaagtccatggttccctggcccgtgctggaaaagtgagaggtcagactcctaag
    >X65921_1787_1912 H.sapiens fau 1 gene
    gtggccaaacaggagaagaagaagaagaagacaggtcgggctaagcggcggatgcagtac
    aaccggcgctttgtcaacgttgtgcccacctttggcaagaagaagggccccaatgccaac
    tcttaa

## Command line arguments

    Extract regions from a sequence
    Version: EMBOSS:6.6.0.0

       Standard (Mandatory) qualifiers:
      [-sequence]          sequence   Sequence filename and optional format, or
                                      reference (input USA)
       -regions            range      [Whole sequence] Regions to extract.
                                      A set of regions is specified by a set of
                                      pairs of positions.
                                      The positions are integers.
                                      They are separated by any non-digit,
                                      non-alpha character.
                                      Examples of region specifications are:
                                      24-45, 56-78
                                      1:45, 67=99;765..888
                                      1,5,8,10,23,45,57,99
      [-outseq]            seqoutall  [<sequence>.<format>] Sequence set(s)
                                      filename and optional format (output USA)

       Additional (Optional) qualifiers:
       -separate           boolean    [N] If this is set true then each specified
                                      region is written out as a separate
                                      sequence. The name of the sequence is
                                      created from the name of the original
                                      sequence with the start and end positions of
                                      the range appended with underscore
                                      characters between them, eg: XYZ region 2 to
                                      34 is written as: XYZ_2_34

       Advanced (Unprompted) qualifiers: (none)
       Associated qualifiers:

       "-sequence" associated qualifiers
       -sbegin1            integer    Start of the sequence to be used
       -send1              integer    End of the sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -scircular1         boolean    Sequence is circular
       -squick1            boolean    Read id and sequence only
       -sformat1           string     Input sequence format
       -iquery1            string     Input query fields or ID list
       -ioffset1           integer    Input start position offset
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-outseq" associated qualifiers
       -osformat2          string     Output seq format
       -osextension2       string     File name extension
       -osname2            string     Base file name
       -osdirectory2       string     Output directory
       -osdbname2          string     Database name to add
       -ossingle2          boolean    Separate file for each entry
       -oufo2              string     UFO features
       -offormat2          string     Features format
       -ofname2            string     Features file name
       -ofdirectory2       string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-sequence] (Parameter 1) | sequence | Sequence filename and optional format, or reference (input USA) | Readable sequence | Required
-regions | range | Regions to extract. A set of regions is specified by a set of pairs of positions. The positions are integers. They are separated by any non-digit, non-alpha character. Examples of region specifications are: 24-45, 56-78 1:45, 67=99;765..888 1,5,8,10,23,45,57,99 | Sequence range | Whole sequence
[-outseq] (Parameter 2) | seqoutall | Sequence set(s) filename and optional format (output USA) | Writeable sequence(s) | <*>.format
Additional (Optional) qualifiers
-separate | boolean | If this is set true then each specified region is written out as a separate sequence. The name of the sequence is created from the name of the original sequence with the start and end positions of the range appended with underscore characters between them, eg: XYZ region 2 to 34 is written as: XYZ_2_34 | Boolean value Yes/No | No
Advanced (Unprompted) qualifiers
(none)
Associated qualifiers
"-sequence" associated sequence qualifiers
-sbegin1 -sbegin_sequence | integer | Start of the sequence to be used | Any integer value | 0
-send1 -send_sequence | integer | End of the sequence to be used | Any integer value | 0
-sreverse1 -sreverse_sequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_sequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_sequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_sequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_sequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_sequence | boolean | Make upper case | Boolean value Yes/No | N
-scircular1 -scircular_sequence | boolean | Sequence is circular | Boolean value Yes/No | N
-squick1 -squick_sequence | boolean | Read id and sequence only | Boolean value Yes/No | N
-sformat1 -sformat_sequence | string | Input sequence format | Any string |
-iquery1 -iquery_sequence | string | Input query fields or ID list | Any string |
-ioffset1 -ioffset_sequence | integer | Input start position offset | Any integer value | 0
-sdbname1 -sdbname_sequence | string | Database name | Any string |
-sid1 -sid_sequence | string | Entryname | Any string |
-ufo1 -ufo_sequence | string | UFO features | Any string |
-fformat1 -fformat_sequence | string | Features format | Any string |
-fopenfile1 -fopenfile_sequence | string | Features file name | Any string |
"-outseq" associated seqoutall qualifiers
-osformat2 -osformat_outseq | string | Output seq format | Any string |
-osextension2 -osextension_outseq | string | File name extension | Any string |
-osname2 -osname_outseq | string | Base file name | Any string |
-osdirectory2 -osdirectory_outseq | string | Output directory | Any string |
-osdbname2 -osdbname_outseq | string | Database name to add | Any string |
-ossingle2 -ossingle_outseq | boolean | Separate file for each entry | Boolean value Yes/No | N
-oufo2 -oufo_outseq | string | UFO features | Any string |
-offormat2 -offormat_outseq | string | Features format | Any string |
-ofname2 -ofname_outseq | string | Features file name | Any string |
-ofdirectory2 -ofdirectory_outseq | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

extractseq reads a single nucleotide or protein sequence.

The input is a standard EMBOSS sequence query (also known as a 'USA').

Major sequence database sources defined as standard in EMBOSS installations include srs:embl, srs:uniprot and ensembl

Data can also be read from sequence output in any supported format written by an EMBOSS or third-party application.

The input format can be specified by using the command-line qualifier `-sformat xxx`, where 'xxx' is replaced by the name of the required format. The available format names are: gff (gff3), gff2, embl (em), genbank (gb, refseq), ddbj, refseqp, pir (nbrf), swissprot (swiss, sw), dasgff and debug.

See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further information on sequence formats.

### Input files for usage example

'tembl:x65923' is a sequence entry in the example nucleic acid database 'tembl'

### Database entry: tembl:x65923

    ID   X65923; SV 1; linear; mRNA; STD; HUM; 518 BP.
    XX
    AC   X65923;
    XX
    DT   13-MAY-1992 (Rel. 31, Created)
    DT   18-APR-2005 (Rel. 83, Last updated, Version 11)
    XX
    DE   H.sapiens fau mRNA
    XX
    KW   fau gene.
    XX
    OS   Homo sapiens (human)
    OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
    OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
    OC   Homo.
    XX
    RN   [1]
    RP   1-518
    RA   Michiels L.M.R.;
    RT   ;
    RL   Submitted (29-APR-1992) to the INSDC.
    RL   L.M.R. Michiels, University of Antwerp, Dept of Biochemistry,
    RL   Universiteisplein 1, 2610 Wilrijk, BELGIUM
    XX
    RN   [2]
    RP   1-518
    RX   PUBMED; 8395683.
    RA   Michiels L., Van der Rauwelaert E., Van Hasselt F., Kas K., Merregaert J.;
    RT   "fau cDNA encodes a ubiquitin-like-S30 fusion protein and is expressed as
    RT   an antisense sequence in the Finkel-Biskis-Reilly murine sarcoma virus";
    RL   Oncogene 8(9):2537-2546(1993).
    XX
    DR   Ensembl-Gn; ENSG00000149806; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000279259; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000434372; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000525297; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000526555; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000527548; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000529259; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000529639; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000531743; Homo_sapiens.
    XX
    FH   Key             Location/Qualifiers
    FH
    FT   source          1..518
    FT                   /organism="Homo sapiens"
    FT                   /chromosome="11q"
    FT                   /map="13"
    FT                   /mol_type="mRNA"
    FT                   /clone_lib="cDNA"
    FT                   /clone="pUIA 631"
    FT                   /tissue_type="placenta"
    FT                   /db_xref="taxon:9606"
    FT   misc_feature    57..278
    FT                   /note="ubiquitin like part"
    FT   CDS             57..458
    FT                   /gene="fau"
    FT                   /db_xref="GDB:135476"
    FT                   /db_xref="GOA:P35544"
    FT                   /db_xref="GOA:P62861"
    FT                   /db_xref="H-InvDB:HIT000322806.14"
    FT                   /db_xref="HGNC:3597"
    FT                   /db_xref="InterPro:IPR000626"
    FT                   /db_xref="InterPro:IPR006846"
    FT                   /db_xref="InterPro:IPR019954"
    FT                   /db_xref="InterPro:IPR019955"
    FT                   /db_xref="InterPro:IPR019956"
    FT                   /db_xref="PDB:2L7R"
    FT                   /db_xref="UniProtKB/Swiss-Prot:P35544"
    FT                   /db_xref="UniProtKB/Swiss-Prot:P62861"
    FT                   /protein_id="CAA46716.1"
    FT                   /translation="MQLFVRAQELHTFEVTGQETVAQIKAHVASLEGIAPEDQVVLLAG
    FT                   APLEDEATLGQCGVEALTTLEVAGRMLGGKVHGSLARAGKVRGQTPKVAKQEKKKKKTG
    FT                   RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
    FT   misc_feature    98..102
    FT                   /note="nucleolar localization signal"
    FT   misc_feature    279..458
    FT                   /note="S30 part"
    FT   polyA_signal    484..489
    FT   polyA_site      509
    XX
    SQ   Sequence 518 BP; 125 A; 139 C; 148 G; 106 T; 0 other;
         ttcctctttc tcgactccat cttcgcggta gctgggaccg ccgttcagtc gccaatatgc        60
         agctctttgt ccgcgcccag gagctacaca ccttcgaggt gaccggccag gaaacggtcg       120
         cccagatcaa ggctcatgta gcctcactgg agggcattgc cccggaagat caagtcgtgc       180
         tcctggcagg cgcgcccctg gaggatgagg ccactctggg ccagtgcggg gtggaggccc       240
         tgactaccct ggaagtagca ggccgcatgc ttggaggtaa agttcatggt tccctggccc       300
         gtgctggaaa agtgagaggt cagactccta aggtggccaa acaggagaag aagaagaaga       360
         agacaggtcg ggctaagcgg cggatgcagt acaaccggcg ctttgtcaac gttgtgccca       420
         cctttggcaa gaagaagggc cccaatgcca actcttaagt cttttgtaat tctggctttc       480
         tctaataaaa aagccactta gttcagtcaa aaaaaaaa                               518
    //

### Input files for usage example 2

### Database entry: tembl:x65921

    ID   X65921; SV 1; linear; genomic DNA; STD; HUM; 2016 BP.
    XX
    AC   X65921; S45242;
    XX
    DT   13-MAY-1992 (Rel. 31, Created)
    DT   14-NOV-2006 (Rel. 89, Last updated, Version 7)
    XX
    DE   H.sapiens fau 1 gene
    XX
    KW   fau 1 gene.
    XX
    OS   Homo sapiens (human)
    OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
    OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
    OC   Homo.
    XX
    RN   [1]
    RP   1-2016
    RA   Kas K.;
    RT   ;
    RL   Submitted (29-APR-1992) to the INSDC.
    RL   K. Kas, University of Antwerp, Dept of Biochemistry T3.22,
    RL   Universiteitsplein 1, 2610 Wilrijk, BELGIUM
    XX
    RN   [2]
    RP   1-2016
    RX   DOI; 10.1016/0006-291X(92)91286-Y.
    RX   PUBMED; 1326960.
    RA   Kas K., Michiels L., Merregaert J.;
    RT   "Genomic structure and expression of the human fau gene: encoding the
    RT   ribosomal protein S30 fused to a ubiquitin-like protein";
    RL   Biochem. Biophys. Res. Commun. 187(2):927-933(1992).
    XX
    DR   Ensembl-Gn; ENSG00000149806; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000279259; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000434372; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000525297; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000526555; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000527548; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000529259; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000529639; Homo_sapiens.
    DR   Ensembl-Tr; ENST00000531743; Homo_sapiens.
    DR   GDB; 191789.
    DR   GDB; 191790.
    DR   GDB; 354872.
    DR   GDB; 4590236.
    XX
    FH   Key             Location/Qualifiers
    FH
    FT   source          1..2016

      [Part of this file has been deleted for brevity]

    FT                   RAKRRMQYNRRFVNVVPTFGKKKGPNANS"
    FT   intron          857..950
    FT                   /number=2
    FT   exon            951..1095
    FT                   /number=3
    FT   intron          1096..1556
    FT                   /number=3
    FT   exon            1557..1612
    FT                   /number=4
    FT   intron          1613..1786
    FT                   /number=4
    FT   exon            1787..>1912
    FT                   /number=5
    FT   polyA_signal    1938..1943
    XX
    SQ   Sequence 2016 BP; 421 A; 562 C; 538 G; 495 T; 0 other;
         ctaccatttt ccctctcgat tctatatgta cactcgggac aagttctcct gatcgaaaac        60
         ggcaaaacta aggccccaag taggaatgcc ttagttttcg gggttaacaa tgattaacac       120
         tgagcctcac acccacgcga tgccctcagc tcctcgctca gcgctctcac caacagccgt       180
         agcccgcagc cccgctggac accggttctc catccccgca gcgtagcccg gaacatggta       240
         gctgccatct ttacctgcta cgccagcctt ctgtgcgcgc aactgtctgg tcccgccccg       300
         tcctgcgcga gctgctgccc aggcaggttc gccggtgcga gcgtaaaggg gcggagctag       360
         gactgccttg ggcggtacaa atagcaggga accgcgcggt cgctcagcag tgacgtgaca       420
         cgcagcccac ggtctgtact gacgcgccct cgcttcttcc tctttctcga ctccatcttc       480
         gcggtagctg ggaccgccgt tcaggtaaga atggggcctt ggctggatcc gaagggcttg       540
         tagcaggttg gctgcggggt cagaaggcgc ggggggaacc gaagaacggg gcctgctccg       600
         tggccctgct ccagtcccta tccgaactcc ttgggaggca ctggccttcc gcacgtgagc       660
         cgccgcgacc accatcccgt cgcgatcgtt tctggaccgc tttccactcc caaatctcct       720
         ttatcccaga gcatttcttg gcttctctta caagccgtct tttctttact cagtcgccaa       780
         tatgcagctc tttgtccgcg cccaggagct acacaccttc gaggtgaccg gccaggaaac       840
         ggtcgcccag atcaaggtaa ggctgcttgg tgcgccctgg gttccatttt cttgtgctct       900
         tcactctcgc ggcccgaggg aacgcttacg agccttatct ttccctgtag gctcatgtag       960
         cctcactgga gggcattgcc ccggaagatc aagtcgtgct cctggcaggc gcgcccctgg      1020
         aggatgaggc cactctgggc cagtgcgggg tggaggccct gactaccctg gaagtagcag      1080
         gccgcatgct tggaggtgag tgagagagga atgttctttg aagtaccggt aagcgtctag      1140
         tgagtgtggg gtgcatagtc ctgacagctg agtgtcacac ctatggtaat agagtacttc      1200
         tcactgtctt cagttcagag tgattcttcc tgtttacatc cctcatgttg aacacagacg      1260
         tccatgggag actgagccag agtgtagttg tatttcagtc acatcacgag atcctagtct      1320
         ggttatcagc ttccacacta aaaattaggt cagaccaggc cccaaagtgc tctataaatt      1380
         agaagctgga agatcctgaa atgaaactta agatttcaag gtcaaatatc tgcaactttg      1440
         ttctcattac ctattgggcg cagcttctct ttaaaggctt gaattgagaa aagaggggtt      1500
         ctgctgggtg gcaccttctt gctcttacct gctggtgcct tcctttccca ctacaggtaa      1560
         agtccatggt tccctggccc gtgctggaaa agtgagaggt cagactccta aggtgagtga      1620
         gagtattagt ggtcatggtg ttaggacttt ttttcctttc acagctaaac caagtccctg      1680
         ggctcttact cggtttgcct tctccctccc tggagatgag cctgagggaa gggatgctag      1740
         gtgtggaaga caggaaccag ggcctgatta accttccctt ctccaggtgg ccaaacagga      1800
         gaagaagaag aagaagacag gtcgggctaa gcggcggatg cagtacaacc ggcgctttgt      1860
         caacgttgtg cccacctttg gcaagaagaa gggccccaat gccaactctt aagtcttttg      1920
         taattctggc tttctctaat aaaaaagcca cttagttcag tcatcgcatt gtttcatctt      1980
         tacttgcaag gcctcaggga gaggtgtgct tctcgg                                2016
    //

You can specify a file of ranges to extract by giving the '-regions' qualifier the value '@' followed by the name of the file containing the ranges. (eg: '-regions @myfile').

The format of the range file is:

- Comment lines start with '#' in the first column.
- Comment lines and blank lines are ignored.
- The line may start with white-space.
- There are two positive (integer) numbers per line separated by one or more space or TAB characters.
- The second number must be greater or equal to the first number.
- There can be optional text after the two numbers to annotate the line.
- White-space before or after the text is removed.

An example range file is:

    # this is my set of ranges
    12   23
     4   5       this is like 12-23, but smaller
    67   10348   interesting region

## Output file format

The output is a standard EMBOSS sequence file.

The results can be output in one of several styles by using the command-line qualifier `-osformat xxx`, where 'xxx' is replaced by the name of the required format. The available format names are: embl, genbank, gff, pir, swiss, dasgff, debug, listfile, dbmotif, diffseq, excel, feattable, motif, nametable, regions, seqtable, simple, srs, table, tagseq.

See: http://emboss.sf.net/docs/themes/SequenceFormats.html for further information on sequence formats.

### Output files for usage example

### File: result.seq

    >X65923 X65923.1 H.sapiens fau mRNA
    ctcgactccat

### Output files for usage example 2

### File: result2.seq

    >X65921 X65921.1 H.sapiens fau 1 gene
    tccctctcgatacactcgggacaagttagggc

If the option `-separate` is used then each specified region is written to the output file as a separate sequence. The name of the sequence is created from the name of the original sequence with the start and end positions of the range appended with underscore characters between them,

For example: "XYZ region 2 to 34" is written as: "XYZ_2_34"

## Data files

None.

## Notes

extractseq allows you to specify one or more regions of a sequence to extract sub-sequences from to build up a contiguous output sequence. This is modelled on the cell's process of splicing out exons from mRNA, but the program is generally applicable to any cutting and splicing or editing operation on a single sequence.

Where the `-regions` option is used to specify output as separate sequences, the name of the sequence is created from the name of the original sequence with the start and end positions of the range appended with underscore characters between them, eg: XYZ region 2 to 34 is written as: `XYZ_2_34`. In such cases the output sequence format must be capable of supporting multiple sequences.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

Several warning messages about malformed region specifications:

- Non-digit found in region ...
- Unpaired start of a region found in ...
- Non-digit found in region ...
- The start of a pair of region positions must be smaller than the end in ...

## Exit status

It exits with status 0, unless a region is badly constructed.

## Known bugs

None noted.

## Comments

## See also

Program name | Description
--- | ---
aligncopy | Read and write alignments
aligncopypair | Read and write pairs from alignments
biosed | Replace or delete sequence sections
codcopy | Copy and reformat a codon usage table
cutseq | Remove a section from a sequence
degapseq | Remove non-alphabetic (e.g. gap) characters from sequences
descseq | Alter the name or description of a sequence
entret | Retrieve sequence entries from flatfile databases and files
extractalign | Extract regions from a sequence alignment
extractfeat | Extract features from sequence(s)
featcopy | Read and write a feature table
featmerge | Merge two overlapping feature tables
featreport | Read and write a feature table
feattext | Return a feature table original text
listor | Write a list file of the logical OR of two sets of sequences
makenucseq | Create random nucleotide sequences
makeprotseq | Create random protein sequences
maskambignuc | Mask all ambiguity characters in nucleotide sequences with N
maskambigprot | Mask all ambiguity characters in protein sequences with X
maskfeat | Write a sequence with masked features
maskseq | Write a sequence with masked regions
newseq | Create a sequence file from a typed-in sequence
nohtml | Remove mark-up (e.g. HTML tags) from an ASCII text file
noreturn | Remove carriage return from ASCII files
nospace | Remove whitespace from an ASCII text file
notab | Replace tabs with spaces in an ASCII text file
notseq | Write to file a subset of an input stream of sequences
nthseq | Write to file a single sequence from an input stream of sequences
nthseqset | Read and write (return) one set of sequences from many
pasteseq | Insert one sequence into another
revseq | Reverse and complement a nucleotide sequence
seqcount | Read and count sequences
seqret | Read and write (return) sequences
seqretsetall | Read and write (return) many sets of sequences
seqretsplit | Read sequences and write them to individual files
sizeseq | Sort sequences by size
skipredundant | Remove redundant sequences from an input set
skipseq | Read and write (return) sequences, skipping first few
splitsource | Split sequence(s) into original source sequences
splitter | Split sequence(s) into smaller sequences
trimest | Remove poly-A tails from nucleotide sequences
trimseq | Remove unwanted characters from start and end of sequence(s)
trimspace | Remove extra whitespace from an ASCII text file
union | Concatenate multiple sequences into a single sequence
vectorstrip | Remove vectors from the ends of nucleotide sequence(s)
yank | Add a sequence reference (a full USA) to a list file

## Author(s)

Gary Williams formerly at:
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.

## History

Written (2000) - Gary Williams

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None